Pollen germination and viability of castor bean (Ricinus communis L.): culture medium composition and environmental conditions
ResumoThe aim of the study was to select the basic components of culture medium and environmental conditions for in vitro pollen germination and viability of castor bean (Ricinus communis L.) of the cultivars IAC 80, AL Guarany 2002 and the hybrid Lara. The four experiments were performed using basic culture medium, where different pH values (5, 6, 7 and 8), incubation temperatures (15, 20, 25 and 30 ºC), sucrose concentrations (0; 5; 10 and 20%) and combinations of boron (4, 8 and 10 mg L-1) with sucrose (0; 5; 10 and 20%) were evaluated. The culture medium was placed in excavated plates and the pollen was distributed on the medium surface. The plates were incubated for one hour. The experimental design was completely randomized and there were 100 pollen grains analyzed in each of the six replications of every treatment. The pH value above 6.0 reduced the amount of pollen grains germinated. Also, temperature of 20 °C and different concentrations of sucrose can be used in germination and pollen tube growth in the castor bean. For all genotypes, the best results concerning the use of boric acid was the 4 mg L-1 combined with 10, 5, and 10% sucrose for the ‘IAC 80’, ‘AL Guarany 2002’ and ‘Lara’, respectively.
Botânica - Botany