Molecular-genetic analysis for validation of peach palm (Bactris gasipaes Kunt) landraces using RAPD markers

Autores

DOI:

https://doi.org/10.15361/1984-5529.2019v47n3p313-320

Palavras-chave:

Análise molecular, Pupunha, Variabilidade genética

Resumo

Numerous landraces of peach palm (Bactris gasipaes var. gasipaes) have been described in the Neotropics and are conserved in a genebank of INPA. We used RAPD markers to analyze the genetic diversity of eight peach palm landraces and two wild populations. Eight primers generated 124 markers; 101 markers were polymorphic (81.5 %). Observed heterozygosity was 0.38 and polymorphism was 93 %, both slightly higher than in previous studies. Amazonian landraces presented high heterozygosity (0.30) and a percentage of polymorphism (87,8 %) similar to Central American landraces (0.29 and 83,5 %, respectively), different from previous studies, which found Central American landraces to have lower values than Amazonian landraces. The structure of the dendrogram with Nei’s genetic distance was similar to that of previous studies, with minor differences. The two wild populations were grouped far from the landraces, suggesting that they did not participate in the domestication of the cultivated populations.

Biografia do Autor

Cirlande Cabral da Silva, Instituto Federal de Educação, Ciência e Tecnologia do Amazonas (IFAM)

Departamento Acadêmico de Educação Básica e Formação de Professores (DAEF)

Doriane Picanço Rodrigues, Universidade Federal do Amazonas (UFAM)

Instituto de Ciências Biológicas, Laboratório de Evolução Aplicada

Spartaco Astolfi Filho, Universidade Federal do Amazonas (UFAM)

Instituto de Ciências Biológicas, Centro de Apoio Multidisciplinar (CAM)

Publicado

09/09/2019

Edição

Seção

Melhoramento Genético Vegetal - Plant Breeding